转录因子Sox-5负调控猪S100A12基因表达

doi:10.11843/j.issn.0366-6964.2014.03.005

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (3): 375-379.doi: 10.11843/j.issn.0366-6964.2014.03.005

转录因子Sox-5负调控猪S100A12基因表达

徐静，唐娟，刘映，赵书红，李新云^*

(华中农业大学动物科技学院农业动物遗传育种与繁殖教育部重点实验室，武汉 430070)

收稿日期:2013-07-05 出版日期:2014-03-23 发布日期:2014-03-23
通讯作者: 李新云，副教授，E-mail:xyli@mail.hzau.edu.cn
作者简介:徐静 (1988-)，女，湖北武汉人，硕士生，主要从事动物遗传育种研究，E-mail:hzauxujing@163.com
基金资助:
教育部新教师基金项目(20090146120032)；国家自然科学基金资助项目(30901020)；863项目（2013AA102502）

The Transcription of S100A12 Gene Negatively Regulated by Sox-5 in Pig

XU Jing，TANG Juan，LIU Ying，ZHAO Shu-hong，LI Xin-yun^*

(Key Laboratory of Agricultural Animal Genetics，Breeding and Reproduction of Ministry of Education，Huazhong Agricultural University，Wuhan 430070， China)

Received:2013-07-05 Online:2014-03-23 Published:2014-03-23

摘要/Abstract

摘要：

本研究旨在确定猪S100A12启动子-1 013~-590区域中起负调控作用的转录因子。首先，通过双荧光素酶报告系统对猪S100A12启动子-1 013~-590区域转录活性进行研究，分别对S100A12启动子-1 013~+598、-931~+598、-830~+598、-711~+598和-590~+598转录活性进行检测。然后对-1 013~-931关键区域进行进一步缺失及突变研究，鉴定该区域负调控S100A12基因的转录因子及其结合位点。结果表明，猪S100A12启动子-1 013~-931区域缺失后，转录活性显著上升，表明该区域存在负调控猪S100A12基因转录因子结合位点。生物信息学手段预测发现，在-1 013~-931区域内存在1个Sox-5，2个GATA-1转录因子结合位点。当转录因子Sox-5结合位点进行缺失及突变时，猪S100A12转录活性显著上升。结果显示转录因子Sox-5负调控猪S100A12基因的表达。

Abstract:

This study aimed to investigate the negative transcriptional factors and their binding motifs in the promoter region of porcine S100A12 gene.The promoter transcriptional activity of the regions，which contains -1 013-+598，-931-+598，-830-+598，-711-+598 and -590-+598 were detected by dual luciferase assay system.Furthermore，the transcriptional activity and the transcriptional factors’ binding sites located in the -1 013--931 region was deeply investigated through DNA deletion and mutation analyses.Porcine S100A12 promoter activity decreased significantly（P<0.05）when the region -1 013--931 was deleted.Bioinformatics analyses results showed that transcriptional factor Sox-5 and GATA-1 binding sites located in this region.The promoter activity was significantly increased only when the binding site of Sox-5 was mutant or deleted.The results indicate that the expression of S100A12 gene is negatively regulated by the transcriptional factor Sox-5.

中图分类号:

S828
S813.1

徐静，唐娟，刘映，赵书红，李新云. 转录因子Sox-5负调控猪S100A12基因表达[J]. 畜牧兽医学报, 2014, 45(3): 375-379.

XU Jing，TANG Juan，LIU Ying，ZHAO Shu-hong，LI Xin-yun. The Transcription of S100A12 Gene Negatively Regulated by Sox-5 in Pig[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 375-379.

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转录因子Sox-5负调控猪S100A12基因表达

The Transcription of S100A12 Gene Negatively Regulated by Sox-5 in Pig

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